Product Name	TSHB
Chinese Name	促甲状腺素β亚单位Recombinant rabbit monoclonal anti
Alias	TSHB_HUMAN; Thyrotropin subunit beta; Thyroid-stimulating hormone subunit beta (TSH-B; TSH-beta); Thyrotropin beta chain; Thyrotropin alfa; TSH-β;
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	D7C6
React Species	Rat
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	12kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] Subcellular Location: Secreted. SWISS: P01222 Gene ID: 7252 Database links: Entrez Gene: 7252 Human SwissProt: P01222 Human TSHB（甲状腺刺激激素亚基Beta）是一种蛋白质编码基因。 与TSHB相关的疾病包括甲状腺功能减退，先天性，非甲状腺功能减退4和中枢性先天性甲状腺功能减退。其相关途径包括通过GPCR进行信号传递和肽激素代谢。与该基因相关的基因本体论（GO）注释包括激素活性。该基因的重要旁系同源物是FSHB。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: Rat pituitary Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 16 kDa Observed MW: 16 kDa Tissue: Rat pituitary Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60432R Cell line: U-2 OS Fixative: 100% methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60432R Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for SLM-60432R

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