Product Name	CLDN1
Chinese Name	紧密连接蛋白1Recombinant rabbit monoclonal anti
Alias	CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1; SEMP1; Senescence-associated epithelial membrane protein; UNQ481/PRO944; ILVASC;
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	B5D10
React Species	Human,Mouse
Applications	WB=1:500-2000 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008] Subcellular Location: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed, with highest levels in liver and kidney. SWISS: O95832 Gene ID: 9076 Database links: Entrez Gene: 9076 Human Entrez Gene: 12737 Mouse SwissProt: O95832 Human SwissProt: O88551 Mouse claudins充当紧密连接复合物的主要成分，该紧密连接复合物调节上皮的通透性。尽管某些claudin家族成员在不可渗透屏障的形成中起着至关重要的作用，但其他一些介导了对离子和小分子的渗透性。通常，几个claudin家族成员会共表达并相互影响，这决定了总体渗透性。 CLDN1是必需的，可防止小分子通过表皮中的紧密连接发生旁细胞扩散，而CLDN1是皮肤的正常屏障功能所必需的。正常的水体内平衡和防止水通过皮肤过多流失可能是必需的，这可能是通过间接影响其他蛋白质的表达水平来实现的，因为CLDN1本身似乎对角质形成细胞紧密连接中的水屏障形成不可或缺。
Product Picture	Sample: Lane 1: Mouse Skin tissue lysates Lane 2: Human HepG2 cell lysates Primary: Anti-CLDN1 (SLM-60433R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: kDa Observed band size: 18 kDa Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HepG2, 2: A431, 3: Mouse liver Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 22 kDa Observed MW: 18 kDa Tissue: Human esophagus Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60433R Tissue: Human colon cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60433R

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