Product Name	MCM5
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	A8E10
React Species	Human,Mouse,Rat
Applications	WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG/Kappa
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	作为MCM2-7复合物（MCM复合物）的组成部分，MCM2-7复合物是真核细胞中“每细胞周期一次”DNA复制起始和延伸所必需的复制螺旋酶。MCM2-7环中的活性ATP酶位点是通过两个相邻亚基的相互作用表面形成的，这样一个保守的精氨酸指基序的关键结构相对于相邻亚基盒的Walker的ATP结合位点反式提供。然而，这六个ATPase活性位点可能对复杂的螺旋酶活性有不同的贡献。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Hela, 2: JK, 3: 4T1 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 82 kDa Observed MW: 82kDa Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60506R Tissue: Rat stomach Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60506R Cell line: Hela Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary Ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary Ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Counter stain: Tubulin (Red) Comment: Color green is the positive signal for SLM-60506R

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