Product Name	Paxillin
Chinese Name
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	E2E8
React Species	Human,Mouse,Rat
Applications	WB=1:500-2000,ICC/IF=1:50-200,Flow-Cyt=1:50-100,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cytoskeleton蛋白参与细胞粘附到Extracellular matrix（粘着斑）位点的肌动蛋白膜附着。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: A431, 3: U87-MG, 4: PC-3, 5: NIH-3T3 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 65 kDa Observed MW: 45-70 kDa Cell line: NIH-3T3 Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60586R Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60586R Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for PTM-6017

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