Product Name	PDCD6IP
Chinese Name	程序性细胞死亡6-相互作用蛋白Recombinant rabbit monoclonal anti
Alias	PDC6I_HUMAN; Programmed cell death 6-interacting protein; AIP1; ALIX; KIAA1375; PDCD6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95;
Research Area	Cell biology Signal transduction Apoptosis transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	R4B8
React Species	Human,Mouse(predicted:Rat)
Applications	WB=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	95 kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PDCD6IP
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012] Subcellular Location: Cytoplasm, cytosol. Melanosome. Cytoplasm, cytoskeleton, centrosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells. SWISS: Q8WUM4 Gene ID: 10015 Database links: Entrez Gene: 10015 Human Entrez Gene: 18571 Mouse Entrez Gene: 501083 Rat SwissProt: Q8WUM4 Human SwissProt: Q9WU78 Mouse SwissProt: Q9QZA2 Rat
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: C6, 3: Mouse brain, 4: Rat brain Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 96 kDa Observed MW: 90/100 kDa Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60716R

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