Product Name	Calreticulin
Chinese Name	钙网蛋白单克隆抗体
Alias	Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.
literatures	Specific References (1) | SLM-51748M has been referenced in 1 publications. [IF=10.383] Qingfei Zhang. et al. Hierarchical Microparticles Delivering Oxaliplatin and NLG919 Nanoprodrugs for Local Chemo-immunotherapy. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXX IF ; Mouse. PubMed:36263713
Research Area	Tumour Signal transduction Cell type markers TumourCell biologyMaker The new supersedes the old
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	G3F7
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	cytoplasmic The cell membrane Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human CALR.
Lsotype	IgG1,κ
Purification	affinity purified by Protein G
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020] Subunit: Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 (By similarity). Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Subcellular Location: Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Similarity: Belongs to the calreticulin family. SWISS: P27797 Gene ID: 811 Database links: Entrez Gene: 811 Human Entrez Gene: 12317 Mouse Entrez Gene: 64202 Rat SwissProt: P27797 Human SwissProt: P14211 Mouse SwissProt: P18418 Rat
Product Picture	Sample: Lane 1: Human SH-SY5Y cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human K562 cell lysates Lane 4: Human HL-60 cell lysates Lane 5: Human A431 cell lysates Lane 6: Human Jurkat cell lysates Lane 7: Human HepG2 cell lysates Lane 8: Human MCF-7 cell lysates Primary: Anti-Calreticulin (SLM-51748M) at 1/1000 dilution Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific Predicted band size: 44 kDa Observed band size: 50 kDa Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Testis tissue lysates Primary: Anti-Calreticulin (SLM-51748M) at 1/1000 dilution Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific Predicted band size: 44 kDa Observed band size: 55 kDa Sample: Lane 1: HepG2 cell lysates Lane 2: Jurkat cell lysates Lane 3: Hela cell lysates Lane 4: MCF-7 cell lysates Lane 5: SH-SY5Y cell lysates Lane 6: K562 cell lysates Lane 7: NIH/3T3 cell lysates Lane 8: C6 cell lysates Lane 9: CHO cell lysates Primary: Anti-Calreticulin (SLM-51748M) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 56 kD Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calreticulin) Monoclonal Antibody, Unconjugated (SLM-51748M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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