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Product Name Propionyl-Histone H4 (Lys12) Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. H4A9 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-1:2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus Form Liquid Concentration 1mg/ml Lsotype IgG Purification Antigen affinity purification Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 组蛋白受多种酶催化修饰,包括乙酰化、甲基化、磷酸化、Ubiquitin化等。赖氨酸丙酰化(Kprop)在结构上与赖氨酸乙酰化相似,是一种新发现的控制蛋白质活性的可逆修饰。赖氨酸丙酰化在原核生物和真核生物中都大量存在,并且已经在包括组蛋白和非组蛋白底物(如p53)的广泛蛋白质中被发现。与组氨酸H3在Lys12位点的乙酰化作用类似,组蛋白H4在Lys12位点的丙酰化作用可能在表观遗传调控中发挥重要作用,包括染色质重塑和转录调控。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+Sodium propionate (10mM, 4hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
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