Product Name	Cpn10
Chinese Name
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	F10C9
React Species	Human,Mouse,Rat
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50-200,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG/Kappa
Purification	Affinity purified by Protein G
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	共伴侣蛋白参与Mitochondrion蛋白质导入和大分子组装。与热休克蛋白60一起，有助于进口蛋白质的正确折叠。还可以防止错误折叠，促进Mitochondrion基质中在应激条件下产生的未折叠多肽的重新折叠和正确组装。这些伴侣蛋白的功能单元由Hsp60大亚基的七聚体环组成，其功能类似于背对背的双环。在一个循环反应中，Hsp60环复合物与每个环上一个未折叠的底物蛋白结合，随后与ATP结合，并与辅伴蛋白Hsp10的两个七聚体环结合。这导致基质蛋白在Hsp60内腔隔离，在一定时间内，它可以折叠而不受其他细胞成分的干扰。所有Hsp60亚基中ATP的同步水解导致伴侣环的解离和ADP和折叠底物蛋白的释放。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate:1: MCF-7, 2: 293, 3: 4T1, 4: PC-12 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 11 kDa Observed MW: 11 kDa Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60471R Tissue: Rat colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60471R Cell line: HeLa Fixative: 100% Ice-cold methanol Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60471R

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