Product Name	PRMT5
Chinese Name	组蛋白精氨酸甲基转移酶5Recombinant rabbit monoclonal anti
Alias	ANM5_HUMAN; Protein arginine N-methyltransferase 5; HRMT1L5; IBP72; JBP1; SKB1; EC:2.1.1.320; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog (SKB1 homolog; SKB1Hs); Protein arginine N-methyltransferase 5, N-terminally processed; protein arginine methyltransferase 5; HSL7; SKB1Hs;
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	D1B12
React Species	Human,Mouse,Rat
Applications	WB=1:500-1000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	70kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG/Kappa
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] SWISS: O14744 Gene ID: 10419 精氨酸甲基转移酶，既能催化omega-N单甲基精氨酸（MMA）和对称二甲基精氨酸（sDMA）的形成，又能优先催化MMA的形成。特异性介导小核核糖核蛋白smd1（SNRPD1）和smd3（SNRPD3）中精氨酸残基的对称二甲基化；这种甲基化是snRNP核心粒子组装和生物生成所必需的。甲基化SUPT5H并可能调节其转录延伸特性。髓鞘碱性蛋白（MBP）精氨酸残基的单酸和二甲基化。可能在cell factor激活的转导途径中发挥作用。负调控细胞周期素E1启动子活性和细胞增殖。在生殖细胞发育过程中甲基化组蛋白H2A和H4'Arg-3'。甲基化组蛋白H3'Arg-8'，可能抑制转录。甲基化Piwi蛋白（PIWIL1，PIWIL2和PIWIL4），Piwi蛋白甲基化是与包含Tudor结构域的蛋白质相互作用和随后定位到减数分裂的细微差别所必需的。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: HepG2 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 73 kDa Observed MW: 70 kDa Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60525R Tissue: Human liver Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60525R

Cartpieces

• 
• 9

  9Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 9

  9Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• A

  ADelete

• 
• A

  ADelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout