Product Name	USP15
Chinese Name
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	H4F4
React Species	Human,Mouse,Rat
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50-200,IF=1:100-500（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG1/Kappa
Purification	Affinity purified by Protein G
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	从靶蛋白中去除结合Ubiquitin并调节多种途径的水解酶，如TGFβ受体信号、NF-κB和RNF41/NRDP1-PRKN途径。作为TGF-β受体信号通路的关键调节因子，但确切机制仍不清楚：根据一份报告，通过促进单Ubiquitin化R-SMADs（SMAD1、SMAD2和/或SMAD3）的去Ubiquitin化而起作用，从而减轻对R-SMADs的抑制并促进TGF-β靶基因的激活。另据报道，通过介导TGFBR1的去Ubiquitin化和稳定化来调节TGF-β受体信号通路，导致TGF-β信号增强。能够介导单Ubiquitin化底物以及“Lys-48”连接的多Ubiquitin链的去Ubiquitin化，保护它们免受蛋白酶体降解。也可能通过组蛋白H2B的去Ubiquitin化调节基因表达和/或DNA修复。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:10000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: HepG2 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 73 kDa Observed MW: 70 kDa

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