Product Name	LY75
Chinese Name	lymphocyte抗原75（CD205）Recombinant rabbit monoclonal anti
Alias	LY75_HUMAN; Lymphocyte antigen 75; CD205; CLEC13B; Ly-75; C-type lectin domain family 13 member B; DEC-205; gp200-MR6;
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	D11H12
React Species	Human,Mouse,Rat
Applications	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,WB=1:500-2000 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	195kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Predicted to enable signaling receptor activity. Predicted to be involved in immune response and inflammatory response. Located in extracellular exosome. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B-lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells. SWISS: O60449 Gene ID: 4065 Database links: Entrez Gene: 4065 Human SwissProt: O60449 Human LY75/DEC-205是一种介导胞吞作用的多凝集素受体，主要表达于树突状细胞和胸腺epithelial cells表面，属于巨噬细胞甘露糖受体家族成员。
Product Picture	Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60593R Cell line: Raji Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Rabbit IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60593R Cell line: Raji Fixative: 4% Paraformaldehyde Permeabilization: 90% Methanol Primary ab dilution: 1:100 Secondary ab: Goat anti Rabbit IgG Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for SLM-60593R

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