Product Name	GOT1
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	H4D9
React Species	Human,Rat(predicted:Mouse)
Applications	WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	从L-天冬氨酸或L-半胱氨酸生物合成L-谷氨酸。谷氨酸水平的重要调节者，谷氨酸是脊椎动物中枢神经系统的主要兴奋性神经递质。在脑神经保护中起到谷氨酸清除剂的作用。天门冬氨酸氨基转移酶活性参与发育过程中肝脏葡萄糖的合成和脂肪细胞的新生。以L-半胱氨酸为底物，调节巯基丙酮酸的水平，巯基丙酮酸是硫化氢的重要来源。巯基丙酮酸通过3-巯基丙酮酸硫转移酶（3MST）的作用转化为H2S。硫化氢是脑内重要的突触调节剂和神经保护剂。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: Mouse heart, 3: Rat heart Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 46 kDa Observed MW: 46 kDa Tissue: Rat kidney Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60599R Tissue: Human cerebrum Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60599R

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