Product Name	MCM3
Chinese Name
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	F4D5
React Species	Human,Mouse,Rat
Applications	WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	充当MCM2-7复合物（MCM复合物）的组成部分，MCM2-7复合物是复制解旋酶，对于真核细胞中的“细胞周期” DNA复制起始和延伸而言是必不可少的。MCM2-7环中的活性ATPase位点是通过两个相邻亚基的相互作用表面形成的，因此相对于相邻的Walker A盒的ATP结合位点，反式提供了一个保守的精氨酸手指基序的关键结构亚基。但是，六个ATPase活性位点可能对复杂的解旋酶活性有不同的贡献, 是DNA复制和细胞增殖所必需。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: K562, 3: HepG2, 4: PC-12, 5: NIH-3T3 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 91 kDa Observed MW: 100 kDa Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60606R Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60606R Tissue: Mouse colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60606R

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