Product Name	PRL
Chinese Name	泌乳素单克隆抗体
Alias	Luteotropic Hormone; Mammotropin; OTTHUMP00000017727; PRL; Prolactin; Prolactin precursor; PRL_HUMAN
Research Area	Cell biology Signal transduction Growth factors and hormones Kinases and Phosphatases Endocrinopathy
Immunogen Species	Mouse
Clonality	Monoclonal
React Species	Human
Applications	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	24kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human PRL protein: 29-227/227
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	PBS
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]. SWISS: P01236 Gene ID: 5617 Database links: Entrez Gene: 5617 Human Entrez Gene: 19109 Mouse Entrez Gene: 24683 Rat Omim: 176760 Human SwissProt: P01236 Human SwissProt: P06879 Mouse SwissProt: P01237 Rat
Product Picture	Sample: Lane 1: Rat Pituitary tissue lysates Primary: Anti-PRL (SLM-43099M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 23 kDa Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRL) Monoclonal Antibody, Unconjugated (SLM-43099M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pituitary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRL ) Monoclonal Antibody, Unconjugated (SLM-43099M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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