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The activity of the buffer in different reaction buffers
Nebuffer 1.1: <10%
Nebuffer 2.0%
Nebuffer 3.1: 100%
Cutsmart buffer: 25%
Features
Restruction enzyme, time -saving enzyme.
The reaction condition
Nebuffer 3.1, 37 ° C.
Heat loss: 65 ° C 20 minutes.
concentration
10,000 and 50,000 units/ml.
Methane sensitivity
is sensitive to mammalian genome DNA CPG methylation.
Note
The NOTL enzyme amount required to thoroughly digest the super -spiral particle is 5 times the digestive linear DNA.