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One Step ELISA Kit For Human Interleukin 8 (IL8)
One Step ELISA Kit For Human Interleukin 8 (IL8)
CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1;il-8
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  • NO.:One-EK012H
    Validity:Two Years
    Assay type:One step sandwich elisa
    Sensitivity:0.43 pg/mL
    Species:Human
    Assay range:4.69 pg/mL - 300 pg/mL
    Assay Time:1.5 hours
    Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
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Details

SAMPLE TYPE Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
SENSITIVITY 0.43 pg/mL
RANGE 15.63 pg/mL - 1000 pg/mL
ASSAY TIME 1.5 h
RECOVERY 89 % - 117 %
AVERAGE RECOVERY 0.97
PLATFORM ELISA
PLATE Detachable 96-well plate
SIZE 96T/48T
STORAGE If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY 4℃ blue ice transportation
COMPONENTS 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL8 monoclonal antibody
Human IL8 freeze-dried standard
IL8 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
ASSAY PRINCIPLE This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Human IL8 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL8 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL8 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL8 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

 

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