SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
SENSITIVITY	0.43 pg/mL
RANGE	4.69 pg/mL - 300 pg/mL
ASSAY TIME	1.5 h
RECOVERY	77 % - 122 %
AVERAGE RECOVERY	0.95
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody Rat TNF-α freeze-dried standard TNF-α detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Rat TNF-α has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for TNF-α (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then TNF-α present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TNF-α bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

 

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