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Avian Interleukin 25 (IL-25)  ELISA Kit

Avian Interleukin 25 (IL-25) ELISA Kit

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  • Cat NO.:SL0064BI
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:1.2 pg/ml - 100 pg/ml
  • Sensitivity:0.1 pg/ml
  • Standard:135 pg/ml
  • Price:$528
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameAvian Interleukin 25 (IL-25) ELISA Kit

Purpose

Our Avian Interleukin 25 (IL-25) ELISA kit is to assay IL-25 levels in Avian serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-25. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-25 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-25 and HRP conjugated IL-25 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-25. You can calculate the concentration of IL-25 in the samples by comparing the OD of the samples to the standard curve.

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