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Human Delta-aminolevulinic Acid Synthase,ALAS ELISA Kit

Human Delta-aminolevulinic Acid Synthase,ALAS ELISA Kit

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  • Cat NO.:SL3231Hu
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:15 pg/ml -400 pg/ml
  • Sensitivity:1 pg/ml
  • Standard:450 pg/ml
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic Name: Human Delta-aminolevulinic Acid Synthase,ALAS ELISA Kit

Purpose

Our Human Delta-aminolevulinic Acid Synthase,ALAS ELISA Kit is to assay ALAS levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ALAS Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for ALAS is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ALAS and HRP conjugated ALAS antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ALAS You can calculate the concentration of ALAS in the samples by comparing the OD of the samples to the standard curve.

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