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Bovine gonadotropin-releasing hormone (GnRH) ELISA Kit

Bovine gonadotropin-releasing hormone (GnRH) ELISA Kit

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  • Cat NO.:SL0196Bo
  • Assay range:6pg/ml -400 pg/ml
  • Sensitivity:1.2 pg/ml
  • Standard:540 pg/ml
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Price:$528
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameBovine gonadotropin-releasing hormone (GnRH) ELISA Kit

Purpose

Our Bovine gonadotropin-releasing hormone (GnRH)ELISA kit is to assay GnRH levels in serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific toGnRH. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for GnRH is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain GnRH and HRP conjugated GnRH antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of GnRH. You can calculate the concentration of GnRH in the samples by comparing the OD of the samples to the standard curve.

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