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Mouse phosphorylated tyrosine-protein kinase (pJAK3) ELISA Kit

Mouse phosphorylated tyrosine-protein kinase (pJAK3) ELISA Kit

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  • Cat NO.:SL0856Mo
  • Product Spec:96T
  • Wavelength:450nm
  • Assay range:3.5 pg/ml -120pg/ml
  • Sensitivity:0.5 pg/ml
  • Standard:135 pg/ml
  • Price:$468
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Drug Names

Generic NameMouse phosphorylated tyrosine-protein kinase pJAK3 (pJAK3)ELISA Kit

Purpose

Our Mouse phosphorylated tyrosine-protein kinase pJAK3 (pJAK3) ELISA kit is to assay pJAK3 levels in Mouse  serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to pJAK3 Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for pJAK3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain pJAK3 and HRP conjugated pJAK3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of pJAK3. You can calculate the concentration of pJAK3 in the samples by comparing the OD of the samples to the standard curve.

 

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