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Porcine Nuclear Factor Kappa B (NF-kB)  ELISA Kit

Porcine Nuclear Factor Kappa B (NF-kB) ELISA Kit

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  • Cat NO.:SL0249Po
  • Product Spec:96T
  • Sample Volume:50ul
  • Wavelength:450nm
  • Assay range:56pg/ml -4000 pg/ml
  • Sensitivity:18pg/ml
  • Standard:5400 pg/ml
  • Price:$528
  • Total:
  • Quantity:
  • Manual

Goods Brief:

Purpose

Our Porcine Nuclear Factor Kappa B (NF-kB)ELISA kit is to assay NF-kB levels in Porcine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to NF-kB Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for NF-kBis added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain NF-kB and HRP conjugated NF-kB antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of NF-kB. You can calculate the concentration of NF-kB in the samples by comparing the OD of the samples to the standard curve.

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