Product Name	Recombinant Rabbit Anti-DIAPH1 Monoclonal Antibody
Cat No.	SLA1914M
Chinese Name	耳聋常染色体显性遗传相关蛋白1重组兔单抗
Alias	DFNA1; DIA1; DRF1; LFHL1; SCBMS; hDIA1; mDia1; D18Wsu154e; Diap1; p140mDia; DIAP1_HUMAN; DIAPH1; Diaphanous-related formin-1 (DRF1); DIAP1_MOUSE; p140mDIA (mDIA1); diaphanous related formin 1; diaphanous (Drosophila, homolog) 1; diaphanous homolog 1 (Drosophila); mammalian diaphanous related formin 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7A10
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Diaphanous homolog 1
Purification	affinity purified by Protein A
Predicted MW.	141 kDa
Observed MW.	160 kDa
Subcellular Location	Cytoplasm
Gene ID	1729
Swiss-Prot	O60610
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with DIAPH1 monoclonal antibody, unconjugated (SLA1914M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DIAPH1 Monoclonal Antibody, Unconjugated (SLA1914M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DIAPH1 Monoclonal Antibody, Unconjugated (SLA1914M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DIAPH1 Monoclonal Antibody, Unconjugated (SLA1914M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DIAPH1 Monoclonal Antibody, Unconjugated (SLA1914M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with DIAPH1 Monoclonal Antibody, Unconjugated (SLA1914M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. The MCF-7 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-DIAPH1 antibody (SLA1914M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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