Product Name	Recombinant Rabbit Anti-GOPC Monoclonal Antibody
Cat No.	SLA1922M
Chinese Name	PDZ结构域蛋白GOPC重组兔单抗
Alias	CAL; FIG; GOPC1; PIST; dJ94G16.2; 2210402P09Rik; GOPC_HUMAN; GOPC; CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10 (PIST); Q3TAF1_MOUSE; GOPC_MOUSE; golgi associated PDZ and coiled-coil motif containing
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	2F3
React Species	Human,Mouse
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human GOPC
Purification	affinity purified by Protein A
Predicted MW.	51 kDa
Observed MW.	60 kDa
Subcellular Location	N/A
Gene ID	57120
Swiss-Prot	Q9HD26
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a role in intracellular protein trafficking and degradation.May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels.May also regulate the intracellular trafficking of the ADR1B receptor.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PIST monoclonal antibody, unconjugated (SLA1922M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (SLA1922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (SLA1922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (SLA1922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (SLA1922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (SLA1922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. The A375 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PIST antibody (SLA1922M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.