Product Name	Recombinant Rabbit Anti-TFEB Monoclonal Antibody
Cat No.	SLA3244M
Alias	TFEB_HUMAN; TFEB; Class E basic helix-loop-helix protein 35 (bHLHe35); BHLHE35; TFEB_MOUSE; Tcfeb; transcription factor EB; Transcription factor EB (Class E basic helix-loop-helix protein 35) (bHLHe35)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5D7
React Species	Human,Mouse,Rat
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Purification	affinity purified by Protein A
Subcellular Location	Nucleus
Gene ID	7942
Swiss-Prot	P19484
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFEB Monoclonal Antibody, Unconjugated (SLA3244M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFEB Monoclonal Antibody, Unconjugated (SLA3244M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFEB Monoclonal Antibody, Unconjugated (SLA3244M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFEB Monoclonal Antibody, Unconjugated (SLA3244M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFEB Monoclonal Antibody, Unconjugated (SLA3244M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TFEB Monoclonal Antibody, Unconjugated (SLA3244M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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