Product Name	Recombinant Rabbit Anti-PLK1 Monoclonal Antibody
Cat No.	SLA3336M
Alias	PLK; STPK13; PLK1_HUMAN; PLK1; Polo-like kinase 1 (PLK-1); Serine/threonine-protein kinase 13 (STPK13); 2.7.11.21; PLK1_MOUSE; PLK1_RAT; polo like kinase 1; polo-like kinase (Drosophila); polo-like kinase 1; Serine/threonine-protein kinase PLK1; PLK-1; Serine/threonine-protein kinase 13
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9A4
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ELISA (1:5000-10000) IP (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Purification	affinity purified by Protein A
Observed MW.	68 kDa
Gene ID	5347
Swiss-Prot	P53350
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PLK1 monoclonal antibody, unconjugated (SLA3336M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (SLA3336M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (SLA3336M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (SLA3336M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (SLA3336M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (SLA3336M) at 1:200 overnight at 4°C, followed by conjugation to SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.