DESCRIPTION

ZBP1 (Z-DNA binding protein 1), also known as DAI or DLM-1, is a nucleic acid sensor that recognises Z-DNA and Z-RNA. Under basal conditions, ZBP1 is highly expressed in human small intestine and lymphoid tissues such as lymph nodes, leukocytes, tonsils, bone marrow and spleen. In mice, it is expressed predominantly in lung, spleen and liver tissues. ZBP1 is involved in the regulation of different types of inflammatory cell death, including necroptosis, pyroptosis, apoptosis and PANoptosis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,237.3	73.0	3.3
2	8	536.2	8.5	1.6
3	8	279.8	5.3	1.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,223.0	67.8	3.1
2	16	538.2	7.9	1.5
3	16	273.5	9.4	3.4

RECOVERY

The recovery of human ZBP1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:8	92	84-104
	1:16	97	94-102

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	86
	Range (%)	85-87
1:8	Average% of Expected	100
	Range (%)	95-105
1:16	Average% of Expected	101
	Range (%)	92-109

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human ZBP1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)