Product Name	CASP3 | Caspase-3 Recombinant Rabbit Monoclonal Antibody
Cat No.	SLA1204M
Product Type	Recombinant Rabbit Monoclonal Antibody, KO/KD Validated
Chinese Name	半胱胺酸蛋白酶蛋白-3重组兔单抗 (for reference only)
React Species	Human, Mouse, Rat
Host Species	Rabbit
Isotype	IgG
Form	Liquid
Clonality	Recombinant
Clone No.	4D2
Immunogen	A synthesized peptide derived from human Caspase 3 (amino acids 176-277 / 277)
Purification	Affinity purified by Protein A
Concentration	1 mg/mL
Theoretical MW	32 kDa
Observed MW	34 kDa
Subcellular Location	Cytoplasm
Gene ID	836 (Human)
Swiss-Prot	P42574 (Human)
Storage Buffer	0.01 M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
Storage	Shipped at 4 ℃. Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.

Applications

Application	Validated Species	Predicted Species	Recommended Dilution
WB	Human, Mouse, Rat	–	1:1000 - 1:5000
IHC-P	Human, Mouse, Rat	–	1:200 - 1:800
IHC-F	Human, Mouse, Rat	–	1:200 - 1:800
IF	Human, Mouse, Rat	–	1:200 - 1:800

Not yet tested in other applications. Optimal working dilutions must be determined by the end user.

Research Areas

Cancer > Cell Death > Apoptosis > Apoptosis Markers > Caspases; Cancer > Cell Death > Apoptosis > Metabolism; Cancer > Invasion/microenvironment > Apoptosis > Caspases; Cell Biology > Apoptosis > Intracellular > Caspases; Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases; Metabolism > Pathways and Processes > Metabolism processes > Apoptosis.

Background

The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Database Links

Entrez Gene: 836 (Human), 12367 (Mouse), 397244 (Pig), 100008840 (Rabbit), 25402 (Rat); OMIM: 600636 (Human); SwissProt: P42574 (Human), P70677 (Mouse), Q95ND5 (Pig), Q8MJC3 (Rabbit), P55213 (Rat); UniGene: 141125 (Human), 34405 (Mouse), 10562 (Rat).

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Attention

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Ordering & shipping

• Minimum order: 1 unit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per unit (25ul / 50ul / 100ul)