Product Name	Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody
Cat No.	SLA1212M
Chinese Name	丝裂原活化蛋白激酶1重组兔单抗
Alias	MK03_HUMAN; MAPK3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1 (ERK-1); Insulin-stimulated MAP2 kinase; MAP kinase isoform p44 (p44-MAPK); Microtubule-associated protein 2 kinase; p44-ERK1; 2.7.11.24; ERK1; PRKM3; MK01_HUMAN; MAPK1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2 (ERK-2); MAP kinase isoform p42 (p42-MAPK); Mitogen-activated protein kinase 2 (MAP kinase 2 | MAPK 2); ERK2; PRKM1; PRKM2; ERK; ERK-2; MAPK2; NS13; P42MAPK; p38; p40; p41; p41mapk; p42-MAPK; ERK-1; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; p44-MAPK; mitogen-activated protein kinase 3
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3B2
React Species	Human
Applications	WB (1:1000-5000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Flow-Cyt (1:100-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ERK1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	44 kDa
Observed MW.	42 kDa
Subcellular Location	Nucleus
Gene ID	5594
Swiss-Prot	P27361
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ERK1 monoclonal antibody, unconjugated (SLA1212M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with ERK1 monoclonal antibody, unconjugated (SLA1212M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1 Monoclonal Antibody, Unconjugated(SLA1212M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1 Monoclonal Antibody, Unconjugated(SLA1212M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1 Monoclonal Antibody, Unconjugated(SLA1212M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1 Monoclonal Antibody, Unconjugated(SLA1212M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ERK1 Monoclonal Antibody, Unconjugated(SLA1212M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining	Figure 8. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ERK1 antibody (SLA1212M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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