DESCRIPTION

ZC3H13 (zinc finger CCCH-type 13-containing protein) is a protein containing a CCCH-type zinc finger structural domain, which is involved in m6A (N6-methyladenosine) modification, and has an important regulatory role in RNA stability, splicing, and translation. ZC3H13 is aberrantly expressed in a variety of cancers, e.g. in hepatocellular carcinoma (HCC), where its downregulated expression correlates with poor prognosis, while overexpression inhibits the tumor cell proliferation and invasion. In colorectal cancer, ZC3H13 inhibits tumor cell proliferation and invasion by suppressing the Ras-ERK signaling pathway. In addition, ZC3H13 plays an important role in cardiovascular diseases, such as in septic cardiomyopathy (SCM), where its expression is up-regulated, and silencing of ZC3H13 increases the expression of proteins related to cell proliferation and iron death, and reduces apoptosis and reactive oxygen species (ROS) accumulation. ZC3H13 has also been associated with neurological disorders, and its aberrant expression may be related to neurodegenerative diseases.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,226.4	82.7	3.7
2	8	510.9	20.1	3.9
3	8	257.4	17.6	6.8

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,231.9	92.6	4.1
2	16	530.9	29.6	5.6
3	16	266.8	17.4	6.5

RECOVERY

The recovery of human ZC3H13 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:16	115	90-129

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	99
	Range (%)	98-100
1:8	Average% of Expected	94
	Range (%)	93-94
1:16	Average% of Expected	105
	Range (%)	101-108

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human ZC3H13 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)