Product Name	Recombinant Rabbit Anti-USP9X Monoclonal Antibody
Cat No.	SLA1902M
Chinese Name	泛素特异性蛋白酶9X重组兔单抗
Alias	DFFRX; FAF; FAF-X; FAM; MRX99; MRXS99F; XLID99; hFAM; 5730589N07Rik; Fafl; RGD1560056; USP9X_HUMAN; USP9X; Deubiquitinating enzyme FAF-X; Fat facets in mammals (hFAM); Fat facets protein-related, X-linked; Ubiquitin thioesterase FAF-X; Ubiquitin-specific protease 9, X chromosome; Ubiquitin-specific-processing protease FAF-X; 3.4.19.12; USP9; USP9X_MOUSE; Fat facets homolog; Ubiquitin carboxyl-terminal hydrolase FAM; ubiquitin specific peptidase 9 X-linked; ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila); ubiquitin specific protease 9, X-linked (fat facets-like, Drosophila); ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila); fat facets-like, X-linked
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human USP9X
Purification	affinity purified by Protein A
Predicted MW.	290 kDa
Observed MW.	310 kDa
Subcellular Location	N/A
Gene ID	8239
Swiss-Prot	Q93008
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins.May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with USP9X monoclonal antibody, unconjugated (SLA1902M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with USP9X Monoclonal Antibody, Unconjugated (SLA1902M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with USP9X Monoclonal Antibody, Unconjugated (SLA1902M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with USP9X Monoclonal Antibody, Unconjugated (SLA1902M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with USP9X Monoclonal Antibody, Unconjugated (SLA1902M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with USP9X Monoclonal Antibody, Unconjugated (SLA1902M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. The 293T (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-USP9X antibody (SLA1902M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.