Product Name	Recombinant Rabbit Anti-PRDX4 Monoclonal Antibody
Cat No.	SLA1847M
Chinese Name	过氧化物还原酶4重组兔单抗
Alias	AOE37-2; AOE372; HEL-S-97n; PRX-4; Prx-iv; Prx4; TRANK; PRDX4_BOVIN; PRDX4; Peroxiredoxin IV (Prx-IV); Thioredoxin-dependent peroxiredoxin 4; 1.11.1.24; PRDX4_HUMAN; Antioxidant enzyme AOE372 (AOE37-2); Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372; PRDX4_MOUSE; Antioxidant enzyme AOE372; PRDX4_RAT; Rno2CysPrx04; peroxiredoxin 4
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1D4
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Peroxiredoxin 4
Purification	affinity purified by Protein A
Predicted MW.	31 kDa
Observed MW.	27 kDa
Gene ID	10549
Swiss-Prot	Q13162
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Peroxiredoxin-4 monoclonal antibody, unconjugated (SLA1847M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-4 Monoclonal Antibody, Unconjugated (SLA1847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-4 Monoclonal Antibody, Unconjugated (SLA1847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-4 Monoclonal Antibody, Unconjugated (SLA1847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-4 Monoclonal Antibody, Unconjugated (SLA1847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-4 Monoclonal Antibody, Unconjugated (SLA1847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Peroxiredoxin-4 Monoclonal Antibody, Unconjugated (SLA1847M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.