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Recombinant Rabbit Anti-THY1 Monoclonal Antibody
Recombinant Rabbit Anti-THY1 Monoclonal Antibody
CD90; CDw90; T25; Thy-1; Thy-1.2; Thy1.1; Thy1.2; CD7; THY1_HUMAN; THY1; Thy-1 antigen; THY1_MOUSE; THY1_RAT; Thy-1 cell surface antigen
Total $200.0
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Regular members: $160.0
  • NO.:SLA1132M
    concentration:1mg/ml
    React Species:Human
    Immunogen Species:Rabbit
    Clonality:Recombinant
    Applications:IHC-P, IHC-F, IF
    Gene name:THY1
    UniProt Entry:P04216
    NCBI Gene Entry:7070
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Product NameRecombinant Rabbit Anti-THY1 Monoclonal Antibody
Cat No.SLA1132M
Chinese NameCD90重组兔单抗
AliasCD90; CDw90; T25; Thy-1; Thy-1.2; Thy1.1; Thy1.2; CD7; THY1_HUMAN; THY1; Thy-1 antigen; THY1_MOUSE; THY1_RAT; Thy-1 cell surface antigen
Host SpeciesRabbit
IsotypeIgG
ClonalityMonoclonal
Clone No.2B7
React SpeciesHuman
ApplicationsIHC-P (1:50-200)
IHC-F (1:50-200)
IF (1:50-200)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
ImmunogenA synthesized peptide derived from human THY1
Purificationaffinity purified by Protein A
Concentration1mg/ml
Predicted MW.18 kDa
Observed MW.25-35 kDa
Subcellular LocationMembrane
Gene ID7070
Swiss-ProtP04216
Storage0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
BackgroundThy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
AttentionThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

SLA1132M Figure 1

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(SLA1132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA1132M Figure 2

Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(SLA1132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA1132M Figure 3

Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(SLA1132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA1132M Figure 4

Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(SLA1132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA1132M Figure 5

Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(SLA1132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA1132M Figure 6

Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(SLA1132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

SLA1132M Figure 7

Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD90/Thy1 Monoclonal Antibody, Unconjugated(SLA1132M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

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