Product Name	Recombinant Rabbit Anti-MUC1 Monoclonal Antibody
Cat No.	SLA0922M
Chinese Name	粘蛋白-1重组兔单抗
Alias	ADMCKD; ADMCKD1; ADTKD2; CA 15-3; CD227; Ca15-3; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM; MUC1_HUMAN; MUC1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3 (CA 15-3); Carcinoma-associated mucin; Episialin; Krebs von den Lungen-6 (KL-6); Peanut-reactive urinary mucin (PUM); Polymorphic epithelial mucin (PEM); Tumor-associated epithelial membrane antigen (EMA); Tumor-associated mucin; MUC1_MOUSE; mucin 1, cell surface associated; mucin 1, transmembrane; medullary cystic kidney disease 1 (autosomal dominant); epithelial membrane antigen
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	SB48
React Species	Human
Applications	WB (1: 500-2000) IHC-P (1: 200-1000) IHC-F (1: 200-1000) IF (1: 200-1000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Synthesized peptide derived from Human MUC1
Purification	Affinity Purification
Observed MW.	350 kDa
Subcellular Location	Nucleus
Gene ID	4582
Swiss-Prot	P15941
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation . MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail. MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation. MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail. Overexpression of MUC1 potentiates transformation and attenuates stress-induced apoptosis through the Akt or p53 pathways
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with MUC1 monoclonal antibody, unconjugated (SLA0922M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(SLA0922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(SLA0922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(SLA0922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(SLA0922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(SLA0922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC1/MUC1/CD227 Monoclonal Antibody, Unconjugated(SLA0922M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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