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Product Name Recombinant Rabbit Anti-VEGFA Monoclonal Antibody Cat No. SLA1057M Chinese Name 血管内皮生长因子重组兔单抗 Alias L-VEGF; MVCD1; VEGF; VPF; VEGF-A; VEGF111; VEGF164; VEGFA_HUMAN; VEGFA; Vascular permeability factor (VPF); VEGFA_MOUSE; VEGFA_RAT; vascular endothelial growth factor A; vascular endothelial growth factor Host Species Rabbit Isotype IgG Clonality Monoclonal Clone No. 4G7 React Species Human,Mouse,Rat Applications IHC-P (1:100-200)
IHC-F (1:100-200)
IF (1:100-200)
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.Immunogen KLH conjugated synthetic peptide derived from human VEGF Purification affinity purified by Protein A Concentration 1mg/ml Predicted MW. 24 kDa Subcellular Location Secreted Gene ID 7422 Swiss-Prot P15692 Storage 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.Background Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature). Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Validation Images
Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VEGFA Monoclonal Antibody, Unconjugated(SLA1057M) at 1:200overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VEGFA Monoclonal Antibody, Unconjugated(SLA1057M) at 1:200overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VEGFA Monoclonal Antibody, Unconjugated(SLA1057M) at 1:200overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VEGFA Monoclonal Antibody, Unconjugated(SLA1057M) at 1:200overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VEGFA Monoclonal Antibody, Unconjugated(SLA1057M) at 1:200overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VEGFA Monoclonal Antibody, Unconjugated(SLA1057M) at 1:200overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with VEGFA Monoclonal Antibody, Unconjugated(SLA1057M) at 1:200overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.
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