Product Name	Recombinant Rabbit Anti-UBA1 Monoclonal Antibody
Cat No.	SLA2993M
Chinese Name	泛素激活酶E1重组兔单抗
Alias	A1S9; A1S9T; A1ST; AMCX1; CFAP124; GXP1; POC20; SMAX2; UBA1A; UBE1; UBE1X; VEXAS; Sbx; Ube-1; UBA1_HUMAN; UBA1; Protein A1S9; Ubiquitin-activating enzyme E1; 6.2.1.45; UBA1_MOUSE; Ubiquitin-activating enzyme E1 X; Ubiquitin-like modifier-activating enzyme 1 X; Ube1ax; UBA1_RAT; ubiquitin like modifier activating enzyme 1; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivity complementing); ubiquitin-like modifier activating enzyme 1; UBA1, ubiquitin-activating enzyme E1 homolog (yeast); POC20 centriolar protein homolog (Chlamydomonas)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5H3
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human UBA1
Purification	affinity purified by Protein A
Predicted MW.	118 kDa
Observed MW.	110-120 kDa
Subcellular Location	Nucleus
Gene ID	7317
Swiss-Prot	P22314
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (UBA1) monoclonal Antibody, unconjugated (SLA2993M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min . DAPI (blue, C02-04002) was used to stain the cell nuclei.	Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with UBA1 monoclonal antibody, unconjugated (SLA2993M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Figure 3. 25 ug total protein per lane of various lysates (see on figure) probed with UBA1 monoclonal antibody, unconjugated (SLA2993M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UBA1 Monoclonal Antibody, Unconjugated (SLA2993M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 13. 4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (UBA1) monoclonal Antibody, unconjugated (SLA2993M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.