Product Name	Recombinant Rabbit Anti-MAPT Monoclonal Antibody
Cat No.	SLA2900M
Chinese Name	磷酸化微管相关蛋白重组兔单抗
Alias	tau; DDPAC; FTD1; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; PPP1R103; Tau-PHF6; tau-40; Mtapt; PHF-tau; MAPT_0N4R; MAPT_1N4R; MAPT_2N4R; RNPTAU; pTau; TAU_BOVIN; MAPT; Neurofibrillary tangle protein; Paired helical filament-tau (PHF-tau); TAU_HUMAN; TAU_MOUSE; TAU_RAT; microtubule associated protein tau; G protein beta1/gamma2 subunit-interacting factor 1; microtubule-associated protein tau, isoform 4; protein phosphatase 1, regulatory subunit 103; Tau-derived paired helical filament hexapeptide; MAPT | Tau (phospho-S404); p-Tau; phospho-Tau; p-MAPT; MAPT | Tau (phospho-Ser404)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	5A16
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Tau around the phosphorylation site of S404
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	79 kDa
Observed MW.	50-65 kDa
Subcellular Location	Cytoplasm
Gene ID	4137
Swiss-Prot	P10636
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Tau proteins are important Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Tau proteins subcellular located in the axons of neurons, in the cytoso l and in association with plasma membrane components. It expressed in neurons. PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with phospho-Tau (S404) monoclonal antibody, unconjugated (SLA2900M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S404) Monoclonal Antibody, Unconjugated(SLA2900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S404) Monoclonal Antibody, Unconjugated(SLA2900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S404) Monoclonal Antibody, Unconjugated(SLA2900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S404) Monoclonal Antibody, Unconjugated(SLA2900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human hippocampus area; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S404) Monoclonal Antibody, Unconjugated(SLA2900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Tau (S404) Monoclonal Antibody, Unconjugated(SLA2900M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MAPT/Tau (S404) Monoclonal Antibody, Unconjugated (SLA2900M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, SL0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.