Product Name	Recombinant Rabbit Anti-H3-3A Monoclonal Antibody
Cat No.	SLA2923M
Chinese Name	磷酸化组蛋白H3.3重组兔单抗
Alias	BRYLIB1; H3.3A; H3F3; H3F3A; BRYLIB2; H3.3B; H3F3B; H3-3b; H33_HUMAN; H3-3A; H33_RAT; H3.3 histone A; H3 histone, family 3A; H3 histone family member 3A; H3-3A | Histone H3.3 (phospho-T3); H3.3T3P; p-Histone H3.3; phospho-Histone H3.3; p-H3.3; H3-3A | Histone H3.3 (phospho-Thr3)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6B8
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Histone H3.3 around the phosphorylation site of T3
Purification	affinity purified by Protein A
Predicted MW.	15 kDa
Observed MW.	15 kDa
Subcellular Location	Nucleus
Gene ID	3020
Swiss-Prot	P84243
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Hela (H) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-Histone H3.3(T3) monoclonal antibody, unconjugated (SLA2923M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (SLA2923M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (SLA2923M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Glioblastoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (SLA2923M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (SLA2923M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (SLA2923M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Histone H3.3(T3) Monoclonal Antibody, Unconjugated (SLA2923M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 8. 4% Paraformaldehyde-fixed HeLa (treated with 100ng/ml of nocodazole for 18 h) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-Histone H3.3(T3)) monoclonal Antibody, unconjugated (SLA2923M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.