DESCRIPTION

CD157/BST1 glycosylphosphatidylinositol-anchored glycoprotein is an evolutionary conserved dual-function receptor and βNAD+-metabolizing ectoenzyme of the ADP-ribosyl cyclases gene family. CD157 is a key player in regulating leukocyte adhesion and migration. It may also affect brain plasticity acting as a molecule at the crossroads of neurogenesis, cerebral angiogenesis, and immune regulation. Evaluation of soluble CD157 (sCD157) in pleural effusions provides a diagnostic aid in malignant mesothelioma. Furthermore, the levels of sCD157 correlate with human peripheral monocyte-derived macrophage bactericidal activity.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,434.9	85.6	6.0
2	8	333.7	25.6	7.7
3	8	164.9	16.4	9.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,406.6	117.9	8.4
2	16	312.0	30.1	9.7
3	16	162.1	14.8	9.1

RECOVERY

The recovery of mouse CD157 spiked to three different levels throughout the range of the assay in lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Lysate	1:64	97	94-100

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The lysate samples were initially diluted 1:16.)

		Lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	107
	Range (%)	104-110
1:8	Average% of Expected	117
	Range (%)	113-121
1:16	Average% of Expected	116
	Range (%)	109-122

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD157 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)