DESCRIPTION

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily. It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages. CD163 mediates endocytosis of haptoglobin-haemoglobin complexes. The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	2,581.2	168.3	6.5
2	20	594.0	15.1	2.5
3	20	148.7	12.1	8.2

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	2,603.0	251.7	9.7
2	24	606.4	59.8	9.9
3	24	141.9	13.9	9.8

RECOVERY

The recovery of mouse CD163 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:256	111	102-129
	1:512	105	96-126
Cell culture supernatant	1:2	91	78-106
	1:4	97	85-118

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum was initially diluted 1:32. )

		Mouse serum	Cell culture supernatant
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	100	102
	Range (%)	98-102	99-107
1:8	Average% of Expected	96	83
	Range (%)	92-99	75-96
1:16	Average% of Expected	95	-
	Range (%)	91-98	-

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD163 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)