Product Name	Recombinant Rabbit Anti-SAMHD1 Monoclonal Antibody
Cat No.	SLA1764M
Chinese Name	单核细胞蛋白5重组兔单抗
Alias	CHBL2; DCIP; HDDC1; MOP-5; SBBI88; hSAMHD1; E330031J07Rik; Mg11; mSAMHD1; SAMH1_HUMAN; SAMHD1; dNTPase; Dendritic cell-derived IFNG-induced protein (DCIP); Monocyte protein 5 (MOP-5); SAM domain and HD domain-containing protein 1 (hSAMHD1); 3.1.5.-; MOP5; SAMH1_MOUSE; Interferon-gamma-inducible protein Mg11; SAM domain and HD domain-containing protein 1 (mSAMHD1); Mg21; SAMH1_DANRE;
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3G4
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-200) IHC-F (1:100-200) IF (1:100-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human SAMHD1
Purification	affinity purified by Protein A
Predicted MW.	72 kDa
Observed MW.	72 kDa
Subcellular Location	N/A
Gene ID	25939
Swiss-Prot	Q9Y3Z3
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks.Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses, such as HIV-1: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells .
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with SAMHD1/MOP5 monoclonal antibody, unconjugated (SLA1764M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SAMHD1/MOP5 Monoclonal Antibody, Unconjugated (SLA1764M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SAMHD1/MOP5 Monoclonal Antibody, Unconjugated (SLA1764M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SAMHD1/MOP5 Monoclonal Antibody, Unconjugated (SLA1764M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SAMHD1/MOP5 Monoclonal Antibody, Unconjugated (SLA1764M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SAMHD1/MOP5 Monoclonal Antibody, Unconjugated (SLA1764M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SAMHD1/MOP5 Monoclonal Antibody, Unconjugated (SLA1764M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. The Daudi (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SAMHD1/MOP5 antibody (SLA1764M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 9. 4% Paraformaldehyde-fixed Daudi (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (SAMHD1/MOP5) monoclonal Antibody, unconjugated (SLA1764M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.