Product Name	Recombinant Rabbit Anti-ACTA1 Monoclonal Antibody
Cat No.	SLA0862M
Chinese Name	肌动蛋白α1重组兔单抗
Alias	ACTA; ASMA; CFTD; CFTD1; CFTDM; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; MPFD; NEM1; NEM2; NEM3; SHPM; Acta-2; Acts; Actsk-1; ACTS_CHICK; ACTA1; Alpha-actin-1; 3.6.4.-; ACTS_BOVIN; ACTS_HUMAN; ACTS_MOUSE; ACTS_PIG; ACTS_RABIT; ACTS_RAT; actin alpha 1, skeletal muscle; nemaline myopathy type 3
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6C11
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human ACTA1
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	42 kDa
Observed MW.	45 kDa
Subcellular Location	Cytoplasm
Gene ID	58
Swiss-Prot	P68133
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Actin monoclonal antibody, unconjugated (SLA0862M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with Actin monoclonal antibody, unconjugated (SLA0862M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min
Figure 3. Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Actin Monoclonal Antibody, Unconjugated (SLA0862M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Actin Monoclonal Antibody, Unconjugated (SLA0862M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Actin Monoclonal Antibody, Unconjugated (SLA0862M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. Paraformaldehyde-fixed, paraffin embedded Mouse tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Actin Monoclonal Antibody, Unconjugated (SLA0862M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 7. Paraformaldehyde-fixed, paraffin embedded Rat tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Actin Monoclonal Antibody, Unconjugated (SLA0862M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 8. The RD (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Actin antibody (SLM-528321R,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed
Figure 9. 4% Paraformaldehyde-fixed RD (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Actin) monoclonal Antibody, unconjugated (SLA0862M) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, SL60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.