Product Name	Rabbit Anti-Cldn1 Polyclonal Antibody
Cat No.	SLA0189
Chinese Name	紧密连接蛋白1抗体
Alias	CLD1; ILVASC; SEMP1; CLD1_HUMAN; CLDN1; Senescence-associated epithelial membrane protein; claudin 1; senescence-associated epithelial membrane protein 1
Host Species	Rabbit
Isotype	IgG
Clonality	Polyclonal
React Species	Human
Applications	WB (1:500-2000) ELISA (1:5000-10000) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant mouse CLDN1 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	23 kDa
Observed MW.	17 kDa
Subcellular Location	Membrane
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CLDN1 polyclonal antibody, unconjugated (SLA0189) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.