Summary:

Purpose

Our Quick Step Dog echinococcus antibody (IgG) ELISA Kit (Quantitative ) is to assay echinococcus-IgG levels in Dog serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to echinococcus-IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for echinococcus-IgG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain echinococcus-IgG and HRP conjugated echinococcus-IgG antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of echinococcus-IgG. You can calculate the concentration of echinococcus-IgG in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0113Ca is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.044	0.0480
Blank Well	0pg/ml	0.052	0.0480
S1	150pg/ml	1.967	1.9630
S1	150pg/ml	1.959	1.9630
S2	100pg/ml	1.328	1.3195
S2	100pg/ml	1.311	1.3195
S3	50pg/ml	0.692	0.7030
S3	50pg/ml	0.714	0.7030
S4	25pg/ml	0.392	0.3875
S4	25pg/ml	0.383	0.3875
S5	12.5pg/ml	0.197	0.1900
S5	12.5pg/ml	0.183	0.1900