Summary:

Purpose

Our Quick Step Canine Cortisol ELISA Kit is to assay Cortisol levels in Canine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Cortisol. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Cortisol is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Cortisol and HRP conjugated Cortisol antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Cortisol. You can calculate the concentration of Cortisol in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0109Ca is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.043	0.0405
Blank Well	0ng/ml	0.038	0.0405
S1	90ng/ml	1.954	1.9615
S1	90ng/ml	1.969	1.9615
S2	60ng/ml	1.318	1.3305
S2	60ng/ml	1.343	1.3305
S3	30ng/ml	0.683	0.6910
S3	30ng/ml	0.699	0.6910
S4	15ng/ml	0.391	0.3865
S4	15ng/ml	0.382	0.3865
S5	7.5ng/ml	0.194	0.1910
S5	7.5ng/ml	0.188	0.1910