Summary:

Purpose

Our Quick Step Canine Kisspeptin 1(KISS1)ELISA Kit is to assay KISS1 levels in Canine serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to KISS1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for KISS1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain KISS1 and HRP conjugated KISS1 Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of KISS1. You can calculate the concentration of KISS1 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0103Ca is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0pg/ml	0.059	0.0560
Blank Well	0pg/ml	0.053	0.0560
S1	360pg/ml	1.975	1.9695
S1	360pg/ml	1.964	1.9695
S2	240pg/ml	1.388	1.3800
S2	240pg/ml	1.372	1.3800
S3	120pg/ml	0.705	0.6995
S3	120pg/ml	0.694	0.6995
S4	60pg/ml	0.383	0.3800
S4	60pg/ml	0.377	0.3800
S5	30pg/ml	0.197	0.2010
S5	30pg/ml	0.205	0.2010