Summary:

Purpose

Our Quick Step Cat Immunoglobulin G (IgG) ELISA Kit is to assay IgG levels in Cat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IgG. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IgG is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IgG and HRP conjugated IgG antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IgG. You can calculate the concentration of IgG in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0015Fe is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ug/ml	0.063	0.0580
Blank Well	0ug/ml	0.053	0.0580
S1	12ug/ml	1.955	1.9585
S1	12ug/ml	1.962	1.9585
S2	8ug/ml	1.288	1.2920
S2	8ug/ml	1.296	1.2920
S3	4ug/ml	0.704	0.7080
S3	4ug/ml	0.712	0.7080
S4	2ug/ml	0.387	0.3965
S4	2ug/ml	0.406	0.3965
S5	1ug/ml	0.196	0.2000
S5	1ug/ml	0.204	0.2000