Summary:

Purpose

Our Quick Step Fish Cholesterol ELISA Kit is to assay Cholesterol levels in Fish serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Cholesterol. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Cholesterol is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Cholesterol and HRP conjugated Cholesterol antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Cholesterol. You can calculate the concentration of Cholesterol in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS0098FI is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Standard	Concentration	OD Value	Average OD Value
Blank Well	0nmol/ml	0.043	0.0460
Blank Well	0nmol/ml	0.049	0.0460
S1	9nmol/ml	1.933	1.9385
S1	9nmol/ml	1.944	1.9385
S2	6nmol/ml	1.394	1.4015
S2	6nmol/ml	1.409	1.4015
S3	3nmol/ml	0.713	0.7095
S3	3nmol/ml	0.706	0.7095
S4	1.5nmol/ml	0.400	0.3970
S4	1.5nmol/ml	0.394	0.3970
S5	0.75nmol/ml	0.206	0.2125
S5	0.75nmol/ml	0.219	0.2125