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Product Name FLII Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. G9C10 React Species Human,Mouse,Rat Applications WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50-200,IF=1:100-500(Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus The cell membrane Form Liquid Concentration 1mg/ml Lsotype IgG2a/Kappa Purification Affinity purified by Protein G Buffer Solution 1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 可能在激素激活核受体(NR)的转录激活中作为辅活化子发挥作用,并与NCOA2和CARM1协同作用。参与雌激素信号传导,参与早期胚胎发育。可能通过抑制Rac1依赖的paxillin磷酸化,在涉及胞质分裂和细胞迁移的Cytoskeleton重排的调节中发挥作用。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: room temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: Jurkat, 2: MCF-7
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 145 kDa
Observed MW: 145 kDa
Tissue: Rat cardiac muscle
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60437M
Tissue: Mouse lung
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60437M
Tissue: Human skeletal muscle
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60437M
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[email protected]
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