Product Name	PTPN2
Chinese Name	酪氨酸蛋白磷酸酶非受体2型单克隆抗体
Alias	PTN2_HUMAN; Tyrosine-protein phosphatase non-receptor type 2; PTPT; EC:3.1.3.48; T-cell protein-tyrosine phosphatase (TCPTP); protein tyrosine phosphatase non-receptor type 2; PTN2; TCPTP; TC-PTP; TCELLPTP;
Research Area	Signal transduction Kinases and Phosphatases
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	F3H2
React Species	Human,Mouse,Rat
Applications	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1:50-100,Flow-Cyt=1:50-100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG1/Kappa
Purification	Affinity purified by Protein G
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011] Subcellular Location: [Isoform 2]: Nucleus. Cytoplasm. Cell membrane. Note=Predominantly localizes to chromatin (By similarity). Able to shuttle between the nucleus and the cytoplasm and to dephosphorylate plasma membrane receptors (PubMed:9488479). Recruited by activated ITGA1 at the plasma membrane. {ECO:0000250, ECO:0000269|PubMed:9488479}. Tissue Specificity: Ubiquitously expressed. Isoform 2 is probably the major isoform. Isoform 1 is expressed in T-cells and in placenta. SWISS: P17706 Gene ID: 5771 Database links: Entrez Gene: 5771 Human Entrez Gene: 19255 Mouse Entrez Gene: 117063 Rat SwissProt: P17706 Human SwissProt: Q06180 Mouse SwissProt: P35233 Rat 去磷酸化受体蛋白酪氨酸激酶的非受体型酪氨酸特异性磷酸酶，包括INSR、EGFR、CSF1R、PDGFR。在The nucleus或细胞质中也去磷酸化非受体蛋白酪氨酸激酶，如JAK1、JAK2、JAK3、Src家族激酶、STAT1、STAT3和STAT6。能够负调控许多信号通路和生物过程，如造血，炎症反应，细胞增殖和分化，葡萄糖稳态。在免疫系统的发育中起着多方面的重要作用，通过FYN和LCK的去磷酸化来控制T细胞的分化和活化，在T细胞受体Signal transduction中发挥作用。去磷酸化CSF1R，负调控其下游信号和巨噬Cell differentiation。通过细胞质激酶JAK1、JAK3及其底物STAT1的去磷酸化负调控cell factor（IL2/白介素-2和Interferon）介导的信号传导，这些激酶在cell factor受体下游传播信号。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: room temperature 2h Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HL-60, A20 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 45-50 kDa Observed MW: 45-50 kDa Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:5000 Primary ab incubation condition: room temperature 2h Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: HepG2 Protein loading quantity: 20 μg Exposure time: 10 s Predicted MW: 45-50 kDa Observed MW: 45-50 kDa Tissue: Human breast cancer Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60446M Tissue: Rat stomach Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60446M Tissue: Mouse liver Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60446M Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color green is the positive signal for SLM-60446M

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